Figure 3

Identification of apoptosis induced by metallic nickel nanoparticles. JB6 cells were seeded onto 24-well plate and incubated overnight. Cells were treated with/without metallic nickel nanoparticles. Continuous monitoring of apoptosis and necrosis was conducted by using a dual fluorescence dye assay after 24 h treatment (A) or 48 h treatment (B).