Figure 8
Light and confocal micrographs of PC12 cells attached to various substrate surfaces. A) Negative control sample with PC12 cells loosely attached to a bare glass surface. B) Positive poly-L-lysine coated glass surface, with numerous attached PC12 cells. C) Light micrograph showing PC12 cells attached to a glass coverslip coated with Sundew adhesive. The coverslip was stained with Alcian Blue to demonstrate the pattern of the coating, and the association of the cells with the scaffold. This micrograph shows a sparse area of attachment that allows clear delineation of individual cells, and a typical coating pattern. Areas with more complete coating had a greater number of attached cells. D) Confocal micrograph displaying a thick area of Sundew adhesive, and the thinner networks branching off from the thickly coated area. The PC12 cells were stained with calcein to determine viability, green equaling viable cells, and it is easy to observe the cells attaching to the Sundew adhesive coated areas. Scale bar = 25 μm.