Figure 4
From: Nanometric self-assembling peptide layers maintain adult hepatocyte phenotype in sandwich cultures
Self-assembling peptide-coated membranes seeded with hepatocytes. (A) Hepatocytes are loaded on top of a thin layer of self-assembling peptide gel on a membrane substrate described in Figure 1. (B) A tissue culture insert is coated with a layer of ~0.5 mm of self-assembling peptides. (C) Gel formation is induced by addition of media, and the inverted cell-seeded membrane from A is placed on top of the equilibrated gel. (D) Finally, the sandwich is covered with media. Therefore, the new sandwich culture system consist of a hydrogel layer at the bottom (~0.5 mm) covered by a thin layer of self-assembling peptide-coated on a porous membrane (PEEK or PTFE). The hepatocytes are placed in within both layers.