Figure 8
From: Nanometric self-assembling peptide layers maintain adult hepatocyte phenotype in sandwich cultures
Expression of hepatocyte markers of cells in sandwich cultures of self-assembling peptide scaffolds and PEEK-WC-PU membranes. (A) Gene expression profile of albumin, CYP3A2, and HNF4-alpha obtained by quantitative PCR relative to gene expression in freshly isolated hepatocytes. Cells cultured on modified PEEK-WC-PU membranes incubated with RAD16-I. (B) Comparison of CYP3A2 gene expression relative to freshly isolated hepatocytes by quantitative PCR with previous results at 7 days of collagen cultures (collagen sandwich) are compared with both self-assembling peptide RAD16-I sandwich cultures (RAD16-I sandwich) and sandwich cultures of self-assembling peptides RAD16-I and PEEK-WC-PU membranes (RAD16-I (PEEK)). Data in A and B is presented as mean ± SD (with statistical significances indicated as ** for p < 0.01 and *** for p < 0.001).