Fig. 1

ICP-MS measurements of Ag(I)(aq) concentrations from Ag ENM oxidative dissolution. Oxidative dissolution of Ag ENMs was compared across four sizes: 10 (black), 20 (red), 30 (blue), and 40 nm (grey). Dissolution of Ag ENMs alone (labeled control) is compared to dissolution in the presence of 50 µM copper sulfate, cyt c, HRP, and Cu(II) azurin. In addition, both 5 and 50 µM Cu(II) azurin with 0.07 mg/ml SPE were reacted with Ag ENMs (labeled [low] SPE), as well as a higher concentration sample with 50 µM Cu(II) azurin with 0.7 mg/ml SPE (labeled [high] SPE). Data for samples with SPE present in solution are shown with the contribution of SPE subtracted from the total Ag(I)(aq) concentration. Raw data for samples with SPE are given in Additional file 1: Figure S1