Contribution of actin filaments and microtubules to cell elongation and alignment depends on the grating depth of microgratings

Table 1 Effect of different substrates on cell alignment

Substrate	FN-lines^a (1 µm line)	1 µm grating^b (0.35 µm deep)	1 µm grating^c (1 µm deep)
Cell alignment (°)^d	10.7 ± 7.7 (n = 106)^e	8.4 ± 7.4 (n = 103)	3.8 ± 3.0 (n = 133)

n cell number
^aFN-lines: TCPS microcontact-printed with FN: 1 µm line and spacing
^b1 μm grating (0.35 μm deep): PDMS gratings (1 µm groove, 1 µm ridge, 0.35 µm deep) coated with FN
^c1 μm grating (1 μm deep): PDMS gratings (1 µm groove, 1 µm ridge, 1 µm deep) coated with FN. n: number of cells
^dCell alignment is defined as the angular difference between the major axis of cells and the long axis of FN-line or each micrograting. It was measured at 12 h after seeding onto each substrate

ISSN: 1477-3155