Fig. 5

Lipo-EF24 blocks I-kappa-B-alpha (IkBa) phosphorylation and NF-kappaB activation. The effect of void liposomes (VL) or Lipo-EF24 (EL) on NF-kappaB pathway activity in a MIAPaCa or b Pa03C cells was assessed by quantifying the protein expression levels of phospho-I-kappa-B-alpha (p-IκBα), I-kappa-B-alpha (IκBα), phospho-NF-kappaB-p65 (p-NFκB p65) and NF-kappaBp-65 (NFκB p65) in cytoplasmic (CE) as well as nuclear (NE) extracts, GAPDH was used as loading control