Synthesis and functionalization of protease-activated nanoparticles with tissue plasminogen activator peptides as targeting moiety and diagnostic tool for pancreatic cancer

Table 4 Flow cytometry analysis

Nanoparticle formulation	Panc-1			Su.86.86
Nanoparticle formulation	2 h	12 h	24 h	2 h	12 h	24 h
t-PApep1_Lac	2.6	87.6	86.3	25.7	45.8	36.6
CAN-Mag@PNPs -tPApep1_Lac	37.3	85.2	74.3	Nd	Nd	Nd
CAN-Mag@PNPs-PEG-REGAcp-PEG/tPApep1_Lac	1.4	2.2	1.8	0.3	0.5	0.9
CAN-Mag@PNPs-PEG-REGAcp-PEG/tPApep1_Lac + MMP9	2.2	Nd	4.1	2.9	4.3	7.8
CAN-Mag-Chitosan–tPApep1_Lac	30.5	63.3	55.3	Nd	Nd	Nd
CAN-Mag-Chitosan-tPApep1_Lac PEG 2 kDa	48.3	52.3	53.5	90.1	91.7	93.7
CAN-Mag-Chitosan–tPApep1_Lac PEG 2&5 kDa	21.8	62.4	68.3	68.9	85.8	79.5
CAN-Mag-chitosan–tPApep-1_Lac PEG 5 kDa	19.5	81.9	86.7	26.3	65.2	66.0

Percentage of cells labeled with nanoparticles (NPs) after 2, 12 and 24 h incubation time. The NP binding was dependent on the NPs composition, the exposure time and cell lines tested
Nd not determined

ISSN: 1477-3155