Fig. 2

ICP-MS measurement of dissolved silver (<3 kDa) in the different culture media. The samples were inoculated with 20 µg/mL nanosilver (a) or 1.5 µg/mL ionic silver (b) and incubated at 37 °C for 48 h. Most of the released silver ions from nanosilver particles, as well as the applied ionic silver formed complexes with the proteins of the culture medium. Next, molecules with molecular weight higher than 3 kDa were removed by cut-off filters and the filtrates of the different culture media, which contained only low molecular weight molecules (<3 kDa), were analyzed by ICP-MS measurement