Technique/probe | Observable | LOD | Application notes | Reference |
---|---|---|---|---|
Direct detection | Absorbance of H2O2 | mM | Â | |
Cyt c | Absorbance | nM | Optimal reaction in low ionic strength solutions | |
Xylenolorange + Fe3+ | Absorbance of complex | μM | Carried out in acidic acids | [59] |
Xylenolorange + Ti4+ | Absorbance of complex | μM | Carried out in acidic acids | [59] |
Luminol | Chemi-luminescence | nM | Interference with Mn2+ and Fe3+ | |
2,7-Dichlorodihydrofluorescein (DCFH) | Fluorescence of product | pM-nM | Can be oxidised by other ROS | |
p-Hydroxyphenylacetic acid | Fluorescence of product | nM | Optimal reaction at pHÂ >Â 8.5 |