Fig. 3

Analysis of the proteolysis resistance of the RICK peptide and the consequence on the PBN stability. a Fluorescence emission of siRNA-Cy3b alone or in the presence of RICK, CADY-K and a control peptide (Ctrl-pep) at a peptide molar ratio of R = 20 in the presence of increasing trypsin amounts. b HPLC chromatograms of RICK and CADY-K in the presence of Trypsin. The dotted square represent the degraded CADY-K. Degradation products are given in Additional file 1: Table S1. c siRNA recovery after serum incubation. Cy3-labeled siRNA alone or complexed to CADY-K or RICK (R = 20) were incubated with serum. siRNAs were extracted from the sample and visualized in an acrylamide gel. Resulting fluorescence intensities were normalized to untreated siRNA (−FBS)