Fig. 2

Preparation and characterization of the biomimetic nanosuspensions. A Detailed procedure for the preparation of DNS-[C6&DC]m. The extracted C6 glioma cancer cell membrane was hybridized with the DC membrane as the shell structure and then coated onto DNS cores using an ultrasonic fusion method. B Morphological appearance of DNS, [C6&DC]m and DNS-[C6&DC]m based on TEM. C C6 glioma cell nuclei obtained by low-speed centrifugation in the cell membrane preparation. D Cell membranes after purification under high speed centrifugation. Hoechst 33,258 (blue), DiI (red), (60 × magnification). E C6 cancer cell membrane was doped with a FRET pair of fluorescent probes and mixed with increasing numbers of DCm. F Confocal fluorescent microscopy images of the DNS-[C6&DC]m or either a mixture of DNS-DCm and DNS-C6m (G) (red = DCm, green = C6m; scale bar = 200 nm). H Particle size distribution of different preparations, as measured using the DLS (n = 3; mean ± SD). I Stability of DNS and DNS-[C6&DC]m J in pH 7.4 PBS and 10% serum-rich media. K Western blot analysis of the expression of membrane-specific proteins, including ICAM, CD44, MHC I, and CD80. All samples were run at equivalent protein concentrations