Fig. 5
Immunomodulatory effects on macrophages of PLNPCu in vitro. a, c The flow cytometric analysis and quantitative results of CD80 (M1 macrophages marker) on RAW 264.7 macrophages after incubation with PLNPCu for different times (3, 6, 24 h). b, d The flow cytometric analysis and quantitative results of CD206 (M2 macrophages marker) on RAW 264.7 macrophages after treatment. e Immunofluorescence examination of RAW 264.7 macrophages after incubation with PLNPCu for 24 h. Scale bars, 50 μm. f, gThe flow cytometric analysis and quantitative results of CD80 and CD206 on M2-macrophages after treatment. Results were expressed as mean ± SD. The significant difference was calculated via one-way ANOVA analysis (c, d, g). (NS represented not significant, *p < 0.05, **p < 0.01, ***p < 0.001)