Fig. 6
From: 3D hanging spheroid plate for high-throughput CAR T cell cytotoxicity assay
Cytotoxicity assay of the mock T and HER2-CAR T cells in spheroids in the 3DHSP. A Optical and fluorescent images of the untreated and treated spheroids of BT474, SKOV3, and U87 cells stained with calcein-AM (green) and ethidium homodimer-1 (red). Scale bar: 200 μm. B Area and green and red fluorescence intensity (C) of the untreated and treated spheroids. Student’s t-test: *p < 0.05, **p < 0.01, ***p < 0.001; NS: not significant