Fig. 3

Distribution and penetration of DiD formulations in vivo. A Biodistribution of DiD signal in stromal-rich xenograft-bearing nude mice treated with free DiD, DiD-L, CFH/DiD-L at predetermined intervals. B The fluorescence images of ex vivo tumors in various formulation groups at 24 h post-administration. The fluorescence intensity of ex vivo (C) tumors and D normal tissues of xenograft-bearing nude mice treated with various formulations at 24 h post-administration. Data are represented as mean ± SD, n = 3, **P < 0.01. E Immunofluorescent section of tumors of tumor-bearing mice treated with free DiD, DiD-L, and CFH/DiD-L at 24 h post-administration. The blue is the nucleus stained by DAPI, the green represents the CAFs labelled with the α-SMA antibody and the red indicates various DiD-labelled formulations