Fig. 1

NPM3 was enriched in BAT and decreased in obesity. A IHC analysis of NPM3 expression in different tissues (WAT, BAT, Liver and Muscle) from 8-week-old male mice, the boxed region was shown at higher magnification at right (scale bar = 50 μm), the expression of NPM3 was pointed by red arrows; B qRT-PCR analysis of relative NPM3 mRNA expressions in ASCs and adipocytes from inguinal adipose tissue (iWAT), epididymal adipose tissue (eWAT) and BAT, data were represented as mean ± SEM and analyzed by one-way ANOVA followed by Tukey’s test, *P < 0.05, **P < 0.01; C AAV-NPM3-EGFP infected BAT was transplanted into the inguinal site of the 8-week old mice and the expressions of EGFP were detected in the iWAT, muscle, liver, lung and kidney after 1 week; D Relationship of plasma NPM3 levels of mice across different bodyweight groups (n = 13) (Linear regression analysis); NPM3 protein expression (n = 3) in iWAT (E), eWAT (F) and BAT (G) of 3-month-old male ob/ob mice and lean mice, data were represented as mean ± SEM and analyzed by student t test, *p < 0.05