Fig. 5

Immunomodulatory function of macrophages in vitro. a Representative flow cytometry results after 1 and 7 d of co-culture with CM of hydrogel systems of RAW264.7. CD 86 represents M1 macrophages surface markers and CD 206 represents M2 macrophages surface markers. b, c Expression of M1-related gene, iNOS, and M2-related genes,Arg-1after the RAW264.7 cells were treated by the day 1 and day 7 CM containing different components for 24 h. d, e ELISA results for TNF-α and IL-10 after the RAW264.7 cells were treated by different CM for 24 h. f In vitro angiogenesis of Ea.hy 926 cells in different MCM and comparison of circle and junction generated in 6 h of culture at 37 ℃(Scale bar: 200 μm). And the quantitative results of h circle and i junction generated were counted manually. g In virto migration of L929 cells were treated by the day 1 and day 7 different MCM containing different components(Scale bar: 100 μm) and j comparison of quantitative results of cell migration ratio in 24 h of culture at 37 ℃. *P < 0.05, **P < 0.01 and ***P < 0.001