Fig. 3

Characterization of lipid-coated albumin nanoparticles (LANP) and detection of siRNA-loaded LANP transfection efficiency. a The average size of albumin-PTX, lipid-coated albumin nanoparticles with PTX (LANP-PTX) and with PTX/SRI-siRNA (LANP-PTX-siSRI) was detected. b The Zeta potential of albumin-PTX, LANP-PTX, and LANP-PTX-siSRI was measured. c The particle size distribution of albumin-PTX and LANP-PTX-siSRI was analyzed. d–e LANP-PTX was photographed by the TEM. f The release of siRNA from LANP-PTX-siSRI into saline was measured. g Migration of blank LANP, Fam-labeled siRNA (Fam-siRNA), and LANP with Fam-labeled siRNA (LANP-Fam-siRNA) in agarose gel electrophoresis. h–i The Fam signals in A2780/PTX and A549/PTX were detected by flow cytometry. j The intracellular distribution of LANP-Fam-siRNA was detected by laser confocal microscopy. Scale bar, 25 μm