Fig. 4
In vitro immune responses mediated by CS@P with laser irradiation. In vitro cell viability of H22 tumor cells incubated with CS@P for 24 h without (a) and with (b) laser irradiation (300 s, 1 W cm−2). Error bars stand for ± SD (n = 3). c Calcein AM/PI staining result of H22 tumor cells after the indicated treatments (C@P concentration: 100 μg mL−1, siRNA 100 nM). d Flow cytometry analysis of apoptosis levels in H22 cells after treatment with PBS, laser, C@P, C@P + laser, CS@P, or CS@P + laser (100 μg/mL C@P, 100 nM siRNA) for 24 h with 808 nm laser irradiation (300 s, 1 W cm−2) or not. e The percentages of mature DCs (CD11c+CD86+CD80+) through flow cytometry after indicated treatments in vitro DCs/H22 co-culture system. Error bars stand for ± SD (n = 3). f The flow cytometry plots and proportions of CD4+ and CD8+ T cells in vitro T lymphocytes/DCs/H22 cells (50:10:1) triple co-culture system (gated on the CD3+). Error bars stand for ± SD (n = 3). g The Treg flow cytometry analysis and frequencies in vitro T lymphocytes/DCs/H22 cells (50:10:1) triple co-culture system. Error bars stand for ± SD (n = 3)