Fig. 4

AMP-mediated siRNA transfection and gene silencing in LPS-induced RAW264.7 cells. A Fluorescence microscopy images of LPS-induced RAW264.7 cells treated with different formulations. Cell nuclei are stained with DAPI (blue). siRNA is labeled with Cy3. Thus, the intracellular siRNA will present red fluorescence signal. Scale bar = 20 μm. B Cell histograms and C Transfection efficiencies for evaluating the siRNA delivery effect by flow cytometry. D The expression levels of TNF-α protein in LPS-induced RAW264.7 cells treated with different formulations were evaluated by the TNF-α ELISA kit. The data are calculated by mean ± SD, n = 3 (*p < 0.05; **p < 0.01; ***p < 0.001). “ns” represents no significant difference