Fig. 2From: Enhanced natural killer cell anti-tumor activity with nanoparticles mediated ferroptosis and potential therapeutic application in prostate cancerFerumoxytol and NK cells synergistically induce ferroptosis in PC3 prostate cancer and the ferumoxytol mediated ferroptosis activates the cytotoxic function of NK cells. A. Confocal images of C11-BODIPY stained PC3 cells showed lipid peroxidation status. PC3 cells were treated with Ferumoxytol and co-cultured with NK-92MI cells (green). (scale bar = 20 µm) B. Fluorescent level of intracellular LPO in PC3 cells treated with each group was measured by flow cytometry. C. NK cell-mediated tumor cell killing effect of each treatment (ferumoxytol, NK-92MI, and ferumoxytol + NK-92MI) was determined by CFSE/7AAD assay. Ferrostatin-1 ferroptosis inhibitor was used to confirm the ferroptosis enhanced NK cell killing efficacy of the co-treatment ferumoxytol + NK-92MI. D. Interferon gamma secretion in only NK cell treatment and ferumoxytol + NK cell treatment. E. Degranulation of NK-92MI cells was determined by analysis of CD107a expression on NK cells. NK-92MI and PC3 cells were co-cultured at a 10:1 effector: target ratio and measured by flow cytometry. The data represent mean ± s.d. (n = 3) and statistical significance was analyzed by two-tailed Student’s t-tests. *P < 0.05, **P < 0.01, and ****P < 0.0001Back to article page