Fig. 2

Knockdown of Rab4 increases the number of MVBs. A Representative TEM images showing the effects of siRab4 or siNC on exosome biogenesis in AML12 cells. Red arrows indicate MVBs. Blue arrows indicate early endosome (EE)/late endosome (LE). B The number of EE and LE per cell profile. Each point represents the number of EE/ LE in each cell profile. C The number of MVBs per cell profile. Each point represents the number of MVBs in each cell profile. D Confocal microscopy analysis of the HRS in AML12 cells transfected with siRab4 or siNC. E Quantification of the number of HRS particles per cell. Each point represents the number of HRS particles from individual cell. All data are expressed as mean \(\pm\) SEM. *p < 0.05