Fig. 3

Technical analysis of density-based EV separation of blood plasma and urine by mass spectrometry-based proteomic analysis (LC-MS/MS). LC-MS/MS data from EV-enriched density fractions 9 and 10 obtained from blood plasma and urine after density-based separation by inexperienced (inexp.), experienced (exp.) operators, and automated liquid handling (auto.) (n = 6) are compared by A coefficients of variation (CV) for protein group quantification, B, C correlation analysis, and D principal component analysis. Correlation is represented as Pearson’s r coefficient in a heatmap and violin plots. **** P < 0.0001, **P < 0.01, *P < 0.05 (Mann–Whitney U test). Source data are provided as Additional file 8: Table S2