Fig. 1

Isolation and characterization of P. aeruginosa-derived SyBV and OMV. a Schematic diagram of the generation of P. aeruginosa SyBV. b Representative TEM images of P. aeruginosa OMV and SyBV. Scale bars, 200 nm. c-e The number of particles derived from 1 mL Luria-Bertani culture medium (c), the number of particles per one microgram of proteins (d), and the total RNA amount included in one OMV and SyBV particle (e; n = 3 independent samples). Data are presented as the mean ± s.e.m. ^**P < 0.01, ^***P < 0.001 by unpaired two-tailed Student’s t-test. f Venn diagram of P. aeruginosa OMV and SyBV proteomes. The mutual proteins are divided into three groups (1.5-fold increase, 1.5-fold decrease, and no change) based on the relative protein abundance (n = 2 independent samples). The up arrow means identified proteins in only SyBV group or 1.5-fold increased proteins in SyBV group compared to OMV. In contrast, the down arrow means identified protein in only OMV group or 1.5-fold decreased proteins in SyBV group compared OMV. g Plot of the log2 value of the relative protein abundance from OMV and SyBV. The solid purple line and dotted lines indicate no change and 1.5-fold change, respectively. h OMV-enriched proteins and SyBV-enriched proteins were analyzed with by GO cellular component annotations