Fig. 4

Comparative analyses of protein signatures of M1- and M2-EVs with protein signatures of their respective parental macrophages. A Venn diagram showing the overlap of protein signatures of M1-EVs with those of M1 BMDMs. B Circos plot depicting the signature proteins and pathways shared between M1-EVs and M1 BMDMs. C Immunoblot analysis of Cxcl10 and Arg1. In each lane, 5 μg proteins from EV lysates were loaded. CD81 served as a loading control. D Overlap of enriched proteins in M2-EVs with those of M2 BMDMs. E Circos plot demonstrating the mutual signature proteins and pathways of M2-EVs and M2 BMDMs. F Heatmap showing that M1-EV signature proteins shared 16 of the top 20 pathways of M1 BMDMs. G Heatmap indicating that M2-EV signature proteins shared 5 of the top 12 pathways of M2 BMDMs