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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Stem cell-derived small extracellular vesicles embedded into methacrylated hyaluronic acid wound dressings accelerate wound repair in a pressure model of diabetic ulcer

Fig. 2

Characterization of MSC-sEVs. (A) NTA: distribution curve with a mean particle diameter of 130 nm. (B) Representative image of MSC-sEVs at TEM. (C) Western blot array for tetraspanin CD63, Epithelial Cell Adhesion Molecule (EpCAM), Annexin A5 (ANXA5), Tumor Susceptibility Gene 101 (TSG101), cis-Golgi matrix protein (GM130), Flotillin 1 (FLOT1), Intercellular Adhesion Molecule 1 (ICAM), programmed cell death 6 interacting protein (ALIX), and tetraspanin CD81. (D) Flow cytometry analysis: MSC-sEVs are positive for CD63 and CD81 exosome surface markers, and for CD90 and CD73 MSC specific surface markers. MSC-sEVs (red pick), EV-depleted medium (gray pick), and vehicle (PBS, black line)

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