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Fig. 1 | Journal of Nanobiotechnology

Fig. 1

From: Mesenchymal stem cells-derived extracellular vesicles protect against oxidative stress-induced xenogeneic biological root injury via adaptive regulation of the PI3K/Akt/NRF2 pathway

Fig. 1

Characterization of extracellular vesicles from human adipose-derived mesenchymal stem cell extracellular vesicles (hASC-EVs). a Transmission electron microscopy (TEM) shows the classic “rim of a cup” and particle vesicle shape. b Nanoparticle tracking analysis (NTA) shows the EV diameter. c Western blot analysis of EVs shows positive results for CD63, TSG101, and HSP70 and negative results for Calnexin. d hASC-EVs taken in by dental follicle cells. The yellow arrow points to PKH26-labeled hASC-EVs. Red: PKH26-labeled EVs; green: FITC-phalloidin; blue: DAPI. Scale bars = 20 µm. e, f ExoView showing CD9, CD63, and CD81 expression in hASC-EVs. Scale bars = 20 μm. g The line graph shows the mean fluorescence intensity (MFI) change of PKH26. (h) PKH26 labeling of EVs shows red coloration, observed microscopically at 0, 3, 6, 12, 24, and 48 h. Scale bars = 50 µm

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Journal of Nanobiotechnology

ISSN: 1477-3155

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