Fig. 3

Incorporation of ropivacaine into hydrogels prolongs the relief of CIPNP. A Schematic diagram of drug administration for chemotherapy in mice. B Mechanical withdrawal threshold in mice was tested at 0, 4, 10, and 24 h after treatment with different hydrogels (n = 6). C p-ERK immunofluorescent staining in the dorsal root ganglion (DRG) of mice at 10 h after the last administration, scale bar = 100 μm. D Statistical results of pERK positive neurons in DRG (n = 6 slices from 3 mice per group). E Schematic diagram of drug administration for chemotherapy in tumor-inoculated mice. F Mechanical withdrawal threshold in tumor-inoculated mice was tested at 0, 4, 10, and 24 h after treatment with different hydrogels (n = 6). G p-ERK immunofluorescent staining in DRG of tumor-inoculated mice at 10 h after the last administration. scale bar = 100 μm. H Statistical results of pERK positive neurons in DRG (n = 6 slices from 3 mice per group). Data are presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. PF: PF127 hydrogel; PFC: cisplatin-loaded PF127 hydrogel; PFC + Rop: cisplatin-loaded PF127 hydrogel plus free ropivacaine; PFCR: cisplatin and ropivacaine-coloaded PF127 hydrogel