Fig. 5
(a) IVONSs:Sm-assisted LDI-MS images of fresh and aged fingerprints in negative-ion mode, and time-dependent MS intensity ratios of (b) OA, (c) AA, and (d) TA. (e) Representative IVONSs:Sm-assisted LDI-MS spectra of fingerprints from a hepatitis patient. The inset shows the corresponding spatial distribution of HA and BilG. (f) The MALDI LIFT-TOF/TOF MS/MS spectrum of BilG obtained from a fingerprint sample in negative-ion mode. (g) Box plot of MS signal intensity at m/z 255.2 and 759.3 between healthy volunteers and hepatitis patients (* P < 0.05). (h) Scattered dot plots of the calculated MS signal intensity ratio (MI759.3/MI255.2) between healthy volunteers and hepatitis patients (* P < 0.01). (i) Comparison between MI759.3/MI255.2 and clinically measured TBil concentration from 6 hepatitis patients (No. 1 ~ 6) and 10 healthy volunteers (No. 7 ~ 16). (j) Correlation between MI759.3/MI255.2 and clinically measured TBil concentration from 6 hepatitis patients and 10 healthy volunteers. Scale bars in (a) and (e) represent 1 mm