Fig. 2

Influence of Ti nanotopographies on the endoplasmic reticulum and FRET between Orai1 and Stim1 in mBMSCs. (A) ER and PM observation in mBMSCs on different surfaces by TEM. (B) Presentative images of FRET efficiency between orai1-GFP and stim1-mCherry with or without CM-4620 on surfaces of PT, NT5, NT20 at 0 s. (C-E) FRET efficiency analysis of the time course of interactions between orai1-GFP and stim1-mCherry with or without CM-4620 on surfaces of PT, NT5, NT20, when the fluorescence was monitored, the 2.5 µM TG was added at 60 s, following by 2mM Calcium at 300s, (F) and the quantification of the area under the curve (AUC). (G) FRET efficiency increase of the time course of orai1-stim1 group compared with NC group between different surfaces (H) and the quantification of the AUC. Data are presented as mean ± SEM, n = 3, Student’s t-test or One-way ANOVA, ns, not significant, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001