Fig. 3

Influence of Ti nanotopographies and stim1 on the migration of single cell. (A) Schematic diagram of observing single cell migration on the surface of titanium samples using laser confocal microscope and assisted by self-made holder. (B) The single cell migration trajectory on different surfaces, and position plots of the cell sites mapped to a common origin, which showed fifteen typical cells in each group. Each site represents the time-dependent position of the center of the nucleus of a single cell. And the quantification of the cell migration distance on different surfaces (n = 22). (C) The single cell migration trajectory after knockdown or overexpression of stim1 on three surfaces and the quantification of the cell migration distance (n = 15). Data are presented as mean ± SEM, Student’s t-test or One-way ANOVA, ns, not significant, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001