Fig. 4

Influence of Ti nanotopographies and stim1 on FAK and Vinculin of mBMSCs. (A) Immunofluorescence staining depicts the expression of vinculin on different surfaces (B) and the quantification of fluorescence intensity of vinculin. (C) Immunofluorescence staining depicts the expression of FAK on different surfaces (D) and the quantification of fluorescence intensity of FAK. (E-H) The expression of vinculin on different surfaces after overexpression (E) or knockdown (G) of stim1 exhibited by immunofluorescence staining, and the quantification of fluorescence intensity of vinculin (F,H). (I-L) The expression of FAK on different surfaces after overexpression (I) or knockdown (K) of stim1 exhibited by immunofluorescence staining, and the quantification of fluorescence intensity of vinculin (J,L). Data are presented as mean ± SEM, n = 3, Student’s t-test or One-way ANOVA, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001