Fig. 5

Influence of Ti nanotopographies and stim1 on the activity of calpain of mBMSCs. (A) mBMSCs on different surfaces were treated with t-BOC-Leu-Met, which is an artificial fluorogenic calpain substrate, for 30 min. And each sample were observed for 10 min, the confocal images at 0 s were exhibited. (B) The time course of fluorescent intensity which represents the time course of calpain activity of mBMSCs on different surfaces. (C) and the quantification of the area under the curve (AUC). (D) mBMSCs overexpressing stim1 on different surfaces were treated with t-BOC-Leu-Met, and each sample were observed for 10 min, the confocal images at 0 s were exhibited. (E-G) The time course of fluorescent intensity of mBMSCs overexpressing stim1 on different surfaces, (H) and the quantification of the AUC. (I) mBMSCs with stim1 knockdown on different surfaces were treated with t-BOC-Leu-Met, and each sample were observed for 10 min, the confocal images at 0 s were exhibited. (J-L) The time course of fluorescent intensity of mBMSCs with stim1 knockdown on different surfaces, (M) and the quantification of the area under the curve (AUC). Data are presented as mean ± SEM, Student’s t-test or One-way ANOVA, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001