Fig. 4

Evaluation of splenic cellular immune responses in immunized mice. A Enumeration of Interferon-gamma (IFN-γ) and Interleukin-4 (IL-4) secreting splenocytes clones re-stimulated with N peptide pool or S1 protein were determined by ELISpot assay. B The levels of IFN-γ, IL-4, and IL-2 secreted in the splenocytes culture medium from immunized mice were detected by cytokine ELISA after stimulation for 2.5 days. C The SI of lymphocyte proliferation assay. D–G Splenocytes were analyzed in FCM. Percentage of activated CD4⁺ (E) and CD8⁺ (G) T cells. D, F Representative pseudocolor plots (smooth on). Bars are presented as mean ± SEM of triplicate assays, where n = 3 mice in each group. Individual values are indicated by colored symbols. Statistical significance was assessed by one-way ANOVA analysis followed by multiple comparisons post hoc tests in A-B, and corresponding P values shown in bar charts. ***p-value < 0.001, **p-value < 0.01, *p-value < 0.05, ^nsp-value > 0.05