Fig. 3
From: Asymmetric adhesive SIS-based wound dressings for therapeutically targeting wound repair
Angiogenesis assays of the SIS/PAA/LAP wound dressing in vitro. a Scheme on preparing the conditioned medium for inducing angiogenic differentiation. b HUVECs scratch assay to estimate cell migration distance. c Semi-quantitative analysis of trace distance based on panel b. d Tube formation assay of HUVECs after 12-hour incubation. e Semi-quantitative analysis of tubule length based on panel d. f Immunofluorescent staining of VEGF (red) /cytoskeleton (green) / DAPI (blue) after 7-day incubation. g Semi-quantitative analysis of VEGF fluorescent intensity based on panel f. N = 4, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001