Fig. 4

CD44 immunofluorescence staining in astrocytes and internalization of Cy5-HA-Se NPs. A Astrocytes stained for CD44 and Dil. Scale bar = 50 μm. B Cy5-HA-Se NPs internalization by astrocytes after 4 and 8 h of incubation. Scale bar = 50 μm. C Targeting efficiency of HA-Se NPs in vivo. Fluorescence images of the major organs after intravenous injection of rats with Cy5-labeled HA-Se NPs. The organs were collected at 24 h post-injection from non-treated rats and at 6, 12, and 24 h post-injection from SCI rats