Fig. 8

Bone regeneration of different scaffolds in vivo. A 3D micro-CT images of different groups. B Calcein-Alizarin Red staining images of different groups. C–E The quantification results of BV/TV, Tb.Th and Tb.Sp at different groups. F The quantification of mineral apposition rate at different groups. G HE staining of different sections. H Masson staining of different sections. I, J Immunochemistry images of OPN and OCN at different groups after implantation for 4 and 12 weeks. K, L The proportion of OPN positive cells at different groups after implantation for 4 and 12 weeks. M, N The proportion of OCN positive cells at different groups after implantation for 4 and 12 weeks. n = 6. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001