Fig. 6
Anti-inflammatory effect of GDNPs in RAW264.7 cells. Changes in intracellular TNF-α (A), IL-6, (B). PGD2 (C), PGE2 (D) and Interleukin 10 (IL-10) (E) mRNA levels in LPS-stimulated RAW264.7 cells treated with and without different doses of GDNPs. The protein levels of Myeloid differentiation primary response gene (88) (MYD88), TLR4, ERK, Phospho-extracellular signal-related kinase (p-ERK), JNK, Phospho-c-Jun N-terminal kinase (p-JNK), p38, p-p38 and β-actin were measured by western blotting in LPS-stimulated RAW264.7 cells after treatment with GDNPs (F). Expression of NO in the presence of LPS and LPS/GDNPs (G). The protein levels of JNK, p-JNK and β-actin were measured by western blotting and compared with the effects of the SP600125 inhibitor (H). Densitometric analysis was performed with ImageJ Software. Data are expressed as mean ± SD. n = 3. #p < 0.05 and ##p < 0.01 vs. Control; *p < 0.05 and **p < 0.01 vs. LPS-stimulated cells (One-way ANOVA and Dunnett’s post-hoc test)