Fig. 9
Effect of GDNPs on inflammatory bowel disease in a mouse model. DSS and GDNPs administration regimens in our IBD mouse model (A). SOD activity in serum (B) and serum MDA levels (C) in IBD mice treated with and without GDNPs. TNF-α (D) and IL-6 (E) mRNA levels in mouse intestinal tissue. Colon length for the different mouse groups (F). Representative photographs of intestines for each mouse group (G). Serum TNF-ɑ (H) and IL-6 (I) detected by ELISA. HE staining of Intestine for each mouse group (J). Protein expression of p-38, p-p38, JNK, p-JNK, GCLM, Nrf2, Keap1, p-p62 and β-actin in intestinal tissue, measured by western blotting (K). Densitometric analysis was performed with ImageJ Software. Data are presented as mean ± SD. n = 3. #p < 0.05 and ##p < 0.01 vs. Control; *p < 0.05 and **p < 0.01 vs. mice treated with DSS only (One-way ANOVA and Dunnett’s post-hoc test)