Table 2 Primers used for the qRT-PCR study (Caco-2)
Gene | Primer (5ʹ to 3ʹ) |
|---|---|
GAPDH | F:CACCAACTGCTTAGCACCCC |
R:TGGTCATGAGTCCTTCCACG | |
TNF-α | F:CCCAGGGACCTCTCTCTAATC |
R:ATGGGCTACAGGCTTGTCACT | |
IL-1β | F:TGCTCAAGTGTCTGAAGCAG |
R:TGGTGGTCGGAGATTCGTAG | |
Claudin-1 | FTGGTCAGGCTCTCTTCACTG |
R:TTGGATAGGGCCTTGGTGTT | |
Occludin | F:AAGGGAAGAGCAGGAAGGTC |
R:TCCAGCTCATCACAGGACTC | |
ZO-1 | F:TTCACGCAGTTACGAGCAAG |
R:TTGGTGTTTGAAGGCAGAGC |