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Table 4 Colonic crypt buffer

From: Ginseng-derived nanoparticles alleviate inflammatory bowel disease via the TLR4/MAPK and p62/Nrf2/Keap1 pathways

Colonic crypt isolation buffer

 

5 * Chelating stock buffer

4 mL

Distilled water

16 mL

Dithiothreitol [100 mM]

100 μL [0.5 mM]

EDTA [0.5 M]

200 μL [5 mM]