Fig. 1

The 3T3-L1 cell viability and fat accumulation after treatment of α-tocopherol, LA, and CLA: (A) the cell viability after treatment of α-tocopherol as determined by MTT assay; (B) the ORO staining of 3T3-L1 cells after treatment of α-tocopherol with MDI stimulation; (C) the ORO quantification of 3T3-LI cells after treatment of α-tocopherol with MDI stimulation; (D) the intracellular TG content of 3T3-LI cells after treatment of α-tocopherol with MDI stimulation; (E) the cell viability after treatment of LA and CLA as determined by MTT assay; (F) the ORO staining of 3T3-L1 cells after treatment of LA and CLA with MDI stimulation; (G) the ORO quantification of 3T3-LI cells after treatment of LA and CLA with MDI stimulation; (H) the ORO staining of 3T3-L1 cells after treatment of combined α-tocopherol and CLA with MDI stimulation; and (I) the ORO quantification of 3T3-LI cells after treatment of combined α-tocopherol and CLA with MDI stimulation. All data are presented as the mean of three experiments ± S.E.M. *** p < 0.001; ** p < 0.01; * p < 0.05 as compared to the control group or MDI-treatment group. ^##p < 0.01, ^#p < 0.05 between the different treatment groups. Scale bar = 100 μm