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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: A xonotlite nanofiber bioactive 3D-printed hydrogel scaffold based on osteo-/angiogenesis and osteoimmune microenvironment remodeling accelerates vascularized bone regeneration

Fig. 5

The influence of CMs on the osteo-/angiogenic differentiation of BMSCs. A, B The ALP staining images and its quantitative assay of BMSCs cultured on CMs for 7 days. C, D The ARS staining and its quantitative assay of BMSCs cultured on CMs for 14 and 21 days. E–G qRT-PCR analysis of BMP-2, OPN and VEGF mRNA expression in BMSCs cultured on CMs for 7 days. H The western blot of the OPN and VEGF e protein expression in BMSCs cultured on CMs for 7 days. I–L The immunofluorescence staining and quantitative assay of the OPN and VEGF protein in BMSCs cultured on CMs for 14 days. *P < 0.05, **P < 0.01 or.***P < 0.001

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Journal of Nanobiotechnology

ISSN: 1477-3155

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