Fig. 6

In vivo evaluation of the 3D-printed scaffolds on vascularized bone regeneration. A Schematic illustration of the surgery procedure. B Micro-CT 3D-reconstructed images of the calvarial defects. C Measurement of BV/TV. D Sequence fluorescence staining images of the calvarial defects. E Percentage of fluorescence labeled regions in D. F Representative photographs of the VG and Masson staining in the calvarial defect areas. G Quantification of the new bone area in F. H H&E staining the calvarial defect areas (the red* denotes the newly formed bone, the yellow ▲ marks the residual biomaterial, and the green arrow marks the capillaries). I The number of newly formed blood vessels in H. ^*P < 0.05, ^**P < 0.01 or.^***P < 0.001