Fig. 3

EMF-USPIO-sEVs restored chondrocyte homeostasis. A-E: Western Blotting bands (A) of MMP13, ADAMTS5, Aggrecan, and COL 2, and corresponding semi-quantifications of the expression levels of MMP13 (B), ADAMTS5 (C), Aggrecan (D) and COL 2 (E). F-I: Relative mRNA expression levels of MMP13 (F), ADAMTS5 (G), Aggrecan (H), and COL 2 (I). J-K: Immunofluorescence images of COL 2 (J) and MMP13 (K) following various treatments (PBS, IL-1, NC-sEVs, USPIO-sEVs, EMF-sEVs, and EMF-USPIO-sEVs). COL2/MMP13 were labeled with FITC (green), and nuclei were labeled with DAPI (blue). *p < 0.05, **p < 0.01, ***p < 0.001 compared to PBS group; #p < 0.05, ##p < 0.01 compared to EMF-sEVs group