Fig. 9

miR-99b-5p exacerbated OA through the MFG-E8/NF- κB pathway. A-D, H-K: Immunofluorescence images of COL 2 (A), MMP13 (B), CD86 (C), and CD206 (D) following various treatments (miR-NC + pcDNA-NC, miR-99b-5p + pcDNA-NC, miR-NC + pcDNA-MFG-E8, and miR-99b-5p + pcDNA-MFG-E8); corresponding semi-quantifications of fluorescence intensity of COL 2 (H), MMP13 (I), CD86 (J) and CD206 (K). COL 2, MMP13, and CD206 were labeled with FITC (green), CD86 was labeled with Cy3 (red), and nuclei were labeled with DAPI (blue). E-G: Western Blotting bands (E) of p-P65 and p-IkB and corresponding semi-quantifications of the expression levels of p-P65 (F) and p-IkB (G). *p < 0.05, **p < 0.01, ***p < 0.001