Fig. 1
Cloning and purification of thermozymes from thermophilic bacteria. A Schematic diagram of the application of recombinant DNA for the protein obtaining from thermophilic bacteria. B PCR amplification of target gene fragments by agarose gel electrophoresis. M: DL2000 DNA marker. Gene length, protein monomer molecular weight and zeta potential are presented in the table (right). C Coomassie brilliant blue staining of proteins following SDS-PAGE. M: molecular weight markers with size indicated in kDa; C: crude extracts of recombinant proteins; P: purified proteins by Ni2+-NTA chromatography