Fig. 3
NIR laser triggered the enzymatic activities of GHE nanocatalyst. A Thermozyme-based GHE nanocatalyst showed enhanced enzymatic activity by light irradiation (left panel) compared to non-irradiation (right panel). Schematic of the hydrolysis of respective amino acids catalyzed by three GHEs upon NIR irradiation. Evaluation of the enzymatic activity of GHE based on B arginase Tli08105, C asparaginase Tli10209 or D asparaginase Ttha0067 in the presence or absence of 808 nm NIR irradiation at 2.0 W/cm2. The bulk temperature in solution was maintained at different gradient. Data are shown as the mean ± SD (n = 3); n.s. represents nonsignificant difference, and **p < 0.01, *p < 0.05 (one-way ANOVA with Tukey’s multiple comparisons test)