Fig. 6

In vitro anticancer efficacy of asparagine deprivation-photothermal-therapeutic performance of GHE-Tli10209/Ttha0067. Induction of cell-proliferation effect in MCF7 cells through the MTT assay by GE/GHE nanocatalysts based on A Tli10209 and B Ttha0067. The thermozyme content was fixed at 25 μg/mL, and equal amounts of GNR/GH contain the same photothermal conversion efficiency compared with GE/GHE (*p < 0.05; **p < 0.01, ***p < 0.001, ****p < 0.0001). Data are shown as the mean ± SD (n = 3). C Flow cytometric quantitative analyses of the endocytosis amounts of the nanocatalysts with different architectures in MCF7 cells for different incubation periods (the nanocatalysts were labeled with FITC). D Flow cytometric quantitative analyses for the apoptosis of Annexin V-FITC/PI co-stained MCF7 cells after co-incubation with different nanocatalysts. E Fluorescence microscopy images of JC-1-labeled MCF7 cells treated with different samples. Scale bar: 200 μm. Cell cycle analysis by flow cytometry in MCF7 for the GHE-photothermal catalytic therapy with PI staining based on F Tli10209 and G Ttha0067. Data are shown as the mean ± SD (n = 3)