Fig. 3

siRNA binding and sensitive release of LFNPs/siRNA complexes. A–C The assays of siRNA condense ability by EB competitive binding, Cy5-siRNA fluorescence quenching, and agarose gel electrophoresis (n = 3). (D) Schematic illustration of ROS/pH-triggered hydrolysis of LFNPs. (E–G) siRNA release profile, particle size, and morphology of LFNPs3-3 complexes incubated with different mediums (n = 3). **P < 0.01 by one-way analysis of variance (ANOVA) with Turkey’s multiple comparisons